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The IFN-y enzyme-linked immunospot (ELI-Spot) assay is often used to map HIV-specific CD8 T-cell responses. We compared overlapping 15-mer pools with optimized CD8 epitopes to screen ELISpot responses in HIV-infected individuals. The 15-mer pools detected responses to previously undefined epitopes, but often missed low-level responses to predefined epitopes, particularly when the epitope was central in the 15-mer, rather than at the N-terminus or C-terminus. These factors should be considered in the monitoring of HIV vaccine trials.

Original publication

DOI

10.1097/01.aids.0000131362.82951.b2

Type

Journal article

Journal

AIDS (London, England)

Publication Date

07/2004

Volume

18

Pages

1595 - 1598

Addresses

MRC Human Immunology Unit, University of Oxford, Oxford, UK.

Keywords

CD8-Positive T-Lymphocytes, Humans, HIV-1, HIV Infections, Epitopes, Enzyme-Linked Immunosorbent Assay, Genes, gag, Interferon-gamma