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The overall degree of complexity of the T cell surface has been unclear, constraining our understanding of its biology. Using global gene expression analysis, we show that 111 of 374 genes encoding well-characterized leukocyte surface antigens are expressed by a resting cytotoxic T cell. Unexpectedly, of 97 stringently defined, T cell-specific transcripts with unknown functions that we identify, none encode proteins with the modular architecture characteristic of 80% of leukocyte surface antigens. Only two encode proteins with membrane topologies found exclusively in cell surface molecules. Our analysis indicates that the cell type-specific composition of the resting CD8+ T cell surface is now largely defined, providing an insight into the overall compositional complexity of the mammalian cell surface and a framework for formulating systematic models of T cell surface-dependent processes, such as T cell receptor triggering.

Original publication

DOI

10.1016/s1074-7613(03)00198-5

Type

Journal article

Journal

Immunity

Publication Date

08/2003

Volume

19

Pages

213 - 223

Addresses

Nuffield Department of Clinical Medicine, The University of Oxford, John Radcliffe Hospital, Headington, Oxford OX3 9DU, United Kingdom.

Keywords

Killer Cells, Natural, T-Lymphocytes, CD4-Positive T-Lymphocytes, CD8-Positive T-Lymphocytes, T-Lymphocytes, Cytotoxic, Clone Cells, Cell Membrane, Humans, Antigens, Surface, Gene Expression, Transcription, Genetic, Gene Library