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Human leukocyte antigen-E (HLA-E) normally presents an HLA class Ia signal peptide to the NKG2A/C-CD94 regulatory receptors on natural killer (NK) cells and T cell subsets. Rhesus macaques immunized with a cytomegalovirus-vectored simian immunodeficiency virus (SIV) vaccine generated Mamu-E (HLA-E homolog)-restricted T cell responses that mediated post-challenge SIV replication arrest in >50% of animals. However, HIV-1-specific, HLA-E-restricted T cells have not been observed in HIV-1-infected individuals. Here, HLA-E-restricted, HIV-1-specific CD8 + T cells were primed in vitro. These T cell clones and allogeneic CD8 + T cells transduced with their T cell receptors suppressed HIV-1 replication in CD4 + T cells in vitro. Vaccine induction of efficacious HLA-E-restricted HIV-1-specific T cells should therefore be possible.

Original publication

DOI

10.1126/sciimmunol.abg1703

Type

Journal article

Journal

Science immunology

Publication Date

03/2021

Volume

6

Addresses

NDM Research Building, Nuffield Department of Medicine, Oxford University, Oxford OX3 7FZ, UK.

Keywords

CD8-Positive T-Lymphocytes, Cell Line, Tumor, Jurkat Cells, Humans, HIV-1, HIV Infections, Peptides, Receptors, Antigen, T-Cell, Histocompatibility Antigens Class I, Epitopes, T-Lymphocyte, Cytokines, Immunophenotyping, Lymphocyte Activation, T-Cell Antigen Receptor Specificity, Amino Acid Sequence, gag Gene Products, Human Immunodeficiency Virus, Host-Pathogen Interactions, Biomarkers