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The heterogeneity and complexity of nonlymphoid tissues has become a major obstacle for the study of immune populations. For this reason, the generation of highly reproducible protocols that allow the analysis of immune cells in these tissues has become crucial for clinical and preclinical research. Here we describe an optimized method that allows the obtention of single-cell suspensions from the skin and lungs to analyze and quantify populations of tissue-resident memory CD8+ T cells by multi-parametric flow cytometry.

Original publication

DOI

10.1007/978-1-4939-8979-9_16

Type

Journal article

Journal

Methods in molecular biology (Clifton, N.J.)

Publication Date

01/2019

Volume

1913

Pages

217 - 222

Addresses

Laboratory of Gene Immunotherapy, Fundación Ciencia & Vida, Santiago, Chile.

Keywords

Lung, CD8-Positive T-Lymphocytes, Skin, Animals, Mice, Flow Cytometry, Cell Separation, Immunologic Memory, Single-Cell Analysis