Single-nanoparticle detection and spectroscopy in cells using a hyperspectral darkfield imaging technique
Fairbairn N., Fernandes R., Carter R., Elliot TJ., Kanaras AG., Muskens OL.
We discuss a hyperspectral darkfield microscopy technique capable of imaging single nanoparticles at biologically compatible (<0.5 W/cm 2) illumination conditions. The microscope was tested on an array of lithographically produced gold nanorod antennas and on colloidal gold nanorods deposited on a glass substrate. As a test for in-vitro imaging capabilities, we studied the uptake of gold nanorods by DC2.4 dendritic cells. © 2013 Copyright SPIE.