Preclinical tumor models have advanced our understanding of the tumor microenvironment. However, the temporal dynamics of cellular recruitment and retention within these models is poorly understood. Here, we present a protocol using transcutaneous labeling of the tumor compartment using subcutaneous and orthotopic tumors. We describe the process of cell line implantation and photoconversion of tumors to differentiate newly recruited cells from those retained within tumors. Photoconversion enables tracking of both immune cell recruitment to tumors and egress to the lymphatics. For complete details on the use and execution of this protocol, please refer to Li et al.1 and Molostvov et al.2.
Journal article
2024-06-01T00:00:00+00:00
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Institute of Immunology and Immunotherapy, The University of Birmingham, B15 2TT Birmingham, UK; Division of Radiotherapy and Imaging, The Institute of Cancer Research, SW3 6JB London, UK; Institute of Cancer and Genomics, The University of Birmingham, B15 2 TT Birmingham, UK. Electronic address: isaac.dean@icr.ac.uk.
Cell Line, Tumor, Animals, Mice, Neoplasms, Cell Tracking, Tumor Microenvironment