AimTo investigate the effects of metformin, dichloroacetate (DCA), and memantine on T98G and U87-MG human glioblastoma (GBM) cells to target tumor cell metabolism in a multi-directional manner.Material and methodsIC50 levels for metformin, DCA, metformin+DCA and memantine were determined by MTT assay in T98G and U87-MG cells in vitro. Casp3, Bcl-2, Bax, c-Myc and GSK-3B protein expressions were investigated post treatments. Fifteen GBM+ tumor tissues were assessed for Casp-3, Bcl-2, Bad, Bax for apoptotic protein expression patterns.ResultsCancer cell metabolism targeting drugs metformin, DCA, metformin+DCA and memantine induced cytotoxicity in a dose-dependent manner in T98G and U87-MG cells. IC50 for memantine is found as 0.5 mM (p < 0.01) which is nearly 10 times lower concentration than that of metformin. Fifteen GBM+ tumor tissues had differential apoptotic protein expressions.ConclusionMemantine exerted anti-cancer mechanism of action in T98G and U87-MG cells, however, such a mechanism requires deeper investigation for GBM treatment.
10.5137/1019-5149.jtn.29176-20.3
Journal article
2021-01-01T00:00:00+00:00
31
233 - 237
4
Gazi University, Faculty of Medicine, Department of Medical Biology and Genetics, Ankara, Turkey.
Cell Line, Tumor, Humans, Glioblastoma, Brain Neoplasms, Metformin, Memantine, Proto-Oncogene Proteins c-bcl-2, Dopamine Agents, Antineoplastic Agents, Hypoglycemic Agents, Apoptosis, Cell Survival, Dose-Response Relationship, Drug, Dichloroacetic Acid